What is microinjection

Monday, May 14, 2012

After completion of the preparation of egg and sperm fertilization stage of culture takes place. Fertilization, standard IVF (in vitro fertilization, IVF) or ICSI (intracytoplasmic sperm injection, ICSI) is provided with. The only difference between IVF and ICSI fertilization phase, it is exactly the same except for the steps. IVF is preferred in patients with non-visible sperm problems. ICSI, the sperm in cases of unexplained infertility problems and are preferred. Washed sperm is placed next to the egg and sperm fertilization IVF performs himself. Unlike the first IVF, ICSI, egg around the fused cells and thus is ready mikroenjeksiyona eggs. After thawing, the cells surrounding the egg, egg maturity is evaluated. There are three types of mature eggs: egg Metaphase-2 (mature eggs) (Figure 1); Metaphase-1 egg (egg mature medium) (Figure 2)-germinal vesicle of eggs (egg immature) (Figure 3). Only the eggs in metaphase-2 is microinjection. Germinal vesicle eggs are not available; Metaphase-1 egg-2 egg during the day can turn into an average 20% of the metaphase and can be used for microinjection.